Phospho-GATA6-Y271 Catalog Number：OM217336

Product Profile

Product NamePhospho-GATA6-Y271Antibody TypePrimary Antibodies

Key Feature

ClonalityPolyclonalIsotypeIgHost SpeciesRabbitTested ApplicationsIFDB

IF~~1:100 DB~~1:100~500:

Species ReactivityHuman

Concentration1 mg/mlPurification

Target Information

Alternative NamesGATA6

Transcription factor GATA-6

GATA-binding factor 6

Molecular Weight(MW)60033 DaFunctionTranscriptional activator that regulates SEMA3C ａnd PLXNA2. Thought to be important for regulating terminal differentiation and/or proliferation

Tissue SpecificityExpressed in heart, gut ａnd gut-derived tissuesCellular LocalizationNucleus.

Database Links

Entrez Gene2627Nucleotide AccessionNP_005248.2

Application

• Application
• Fluorescent confocal image of HepG2 cells stained with phospho-GATA6-Y271 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3766a phospho-GATA6-Y271 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-GATA6 immunosignal mainly to the nucleus.

• Application
• Dot blot analysis of Phospho-GATA6-pY271 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Application NotesIF~~1:100 DB~~1:100~500:

Additional Information

FormLiquidStorage InstructionsFor short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.Storage BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

標題：Phospho-GATA6-Y271 Catalog Number：OM217336

地址：https://www.vogueseek.com/post/83996.html

鄭重聲明：本文版權歸原作者所有，轉載文章僅為傳播信息之目的，不構成任何投資建議，如有侵權行為，請第一時間聯絡我們修改或刪除，多謝。